Wrapping Petri Dishes in Parafilm

Parafilm is a flexible film, available in several different lengths and widths. It is commonly used for sealing or protecting containers, like our Petri dishes. It will help to ensure that our samples do not become contaminated by outside substances.

First, don’t get frustrated if the parafilm rips, and realize that this is a technique that improves with practice!

Figure 3.38: Start with about 2.5 squares long piece. Peel off the paper covering. Image by Dr. Marisa Pedulla.

Figure 3.38: Start with about 2.5 squares long piece. Peel off the paper covering.
Image by Dr. Marisa Pedulla.

Figure 3.39: Holding the Petri dish between your thumb and middle, ring and pinkey fingers, use your index finger to pinch one end of the parafilm to the top and bottom of the Petri dish (covering the opening). Image by Dr. Marisa Pedulla.

Figure 3.39: Holding the Petri dish between your thumb and middle, ring and pinkey fingers, use your index finger to pinch one end of the parafilm to the top and bottom of the Petri dish (covering the opening).
Image by Dr. Marisa Pedulla.

Figure 3.40: Using your other hand and starting near the pinched end, stretch the parafilm evenly. Image by Dr. Marisa Pedulla.

Figure 3.40: Using your other hand and starting near the pinched end, stretch the parafilm evenly.
Image by Dr. Marisa Pedulla.

Figure 3.41: Slowly rotate the Petri dish in your while continuing to stretch the parafilm. Image by Dr. Marisa Pedulla.

Figure 3.41: Slowly rotate the Petri dish in your while continuing to stretch the parafilm.
Image by Dr. Marisa Pedulla.

Figure 3.42: Continue rotating and stretching until the parafilm overlaps itself. Image by Dr. Marisa Pedulla.

Figure 3.42: Continue rotating and stretching until the parafilm overlaps itself.
Image by Dr. Marisa Pedulla.

Figure 3.43: Gently push on the overlapping parafilm to make a seal. Image by Dr. Marisa Pedulla.

Figure 3.43: Gently push on the overlapping parafilm to make a seal.
Image by Dr. Marisa Pedulla.

 

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One Response to “Wrapping Petri Dishes in Parafilm”
  1. jt beatty says:

    Have you noticed a decline in the quality of parafilm? It has changed such that I can no longer stretch the film like I used to….

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The MSP project is funded by an ESEA, Title II Part B Mathematics and Science Partnership Grant through the Montana Office of Public Instruction. MSP was developed by the Clark Fork Watershed Education Program and faculty from Montana Tech of The University of Montana and Montana State University, with support from other Montana University System Faculty.